Microbial contamination on operating room surfaces: A cross-sectional study
DOI:
https://doi.org/10.5327/Z1414-44251016%20Keywords:
Operating rooms, Cross infection, Bacteria. Fungi, ContaminationAbstract
Objective: To analyze microbial contamination on operating room surfaces. Method: A cross-sectional study was conducted using sampling from surgical procedures in specialties with high surgical site infection rates. Sterile swabs were collected from furniture and equipment immediately after patient discharge, prior to concurrent cleaning. Results: Twelve surgeries were analyzed, considering variables such as surgical specialty and procedure classification. Bacterial cultures showed that the surgical site had 100% positivity, followed by the surgical table with 91.67%. For fungi, the preparation table and anesthesia equipment showed 75% positivity. Conclusion: The results provide relevant insights into the distribution and prevalence of microorganisms on furniture and equipment surfaces used in surgical procedures, contributing to a better understanding of microbiology in this context.
References
Motta ROL, Oliveira ML, Azevedo SL. Contribuição da Teoria Ambientalista de Florence Nightingale no controle das infecções hospitalares. Rev Multidiscip Saúde. 2021;2(3):112. https://doi.org/10.51161/rems/1524
Association of Perioperative Registered Nurses. Guidelines for perioperative practice. Denver: AORN; 2017.
Sociedade Brasileira de Enfermeiros de Centro Cirúrgico. Práticas recomendadas: centro cirúrgico, recuperação pós-anestésica e centro de material e esterilização. 8a ed. São Paulo: SOBECC; 2021.
Wohrley JD, Bartlett AH. The role of the environment and colonization in healthcare-associated infections. In: McNeil J, Campbell J, Crews J, editors. Healthcare-associated infections in children. Cham: Springer; 2019. https://doi.org/10.1007/978-3-319-98122-2_2
Centers for Disease Control and Prevention. Surgical site infection event (SSI) [Internet]. 2020 [accessed on Feb. 10, 2024]. Available at: www.cdc.gov/nhsn/pdfs/pscmanual/9pscssicurrent.pdf
Dall’olio L, Raggi A, Sanna T, Mazzetti M, Orsi A, Zanni A, et al. Surveillance of environmental and procedural measures of infection control in the operating theatre setting. Int J Environ Res Public Health. 2017;15(1):46. https://doi.org/10.3390/ijerph15010046
Paula CC, Passos JPC, Paula CAVL, Shimoya-Bittencourt W. Análise microbiológica de superfícies inanimadas de Unidades de terapia intensiva em hospital privado e público. Saúde (Santa Maria). 2024;50(1):e85372. https://doi.org/10.5902/2236583485372
Procop GW, Church DL, Hall GS, Janda WM, Koneman EW, Schreckenberger PC, et al. Diagnóstico microbiológico: texto e atlas colorido. 7a ed. Rio de Janeiro: Guanabara Koogan; 2017.
Pádua DG, Silva RVS, Silva JM, Martins CC, Mendes ECB, Kozusny-Andreani DI. Análise de contaminação em superfícies inanimadas de diferentes setores do hospital. Rev Funec Cient. 2019;7(9). https://doi.org/10.24980/rfcm.v7i9.3384
Vaz DWN, Sousa YM, Cavalcante RL, Almeida MGC, Lima KVB, Silva Júnior AF. Avaliação microbiológica dos leitos cirúrgicos de um hospital público oncológico da região norte do Brasil. Res Soc Dev. 2021;10(1):e16610111602. https://doi.org/10.33448/rsd-v10i1.11602
Fukada T, Iwakiri H, Ozaki M. Anaesthetists’ role in computer keyboard contamination in an operating room. J Hosp Infect. 2008;70(2):148-53. https://doi.org/10.1016/j.jhin.2008.05.023
Dresch F, Birkheuer CF, Rempel C, Maciel MJ. Contaminação de superfícies localizadas em unidades de terapia intensiva e salas de cirurgia: uma revisão sistemática da literatura. Rev Epidemiol Control Infec. 2018;8(1):85-91. https://doi.org/10.17058/reci.v1i1.9897
Brasil. Agência Nacional de Vigilância Sanitária. Segurança do paciente em serviços de saúde: limpeza e desinfecção de superfícies [Internet]. Brasília: Anvisa; 2010 [accessed on Feb. 08, 2024]. Available at: https://www.gov.br/anvisa/pt-br/centraisdeconteudo/publicacoes/servicosdesaude/publicacoes/manual-de-limpeza-e-desinfeccao-de-superficies.pdf/view
Nascimento EAS, Poveda VB, Monteiro J. Evaluation of different monitoring methods of surface cleanliness in operating rooms. Rev Bras Enferm. 2021;74(3):e20201263. https://doi.org/10.1590/0034-7167-2020-1263
Pasquarella C, Balocco C, Colucci ME, Saccani E, Paroni S, Albertini L, et al. The influence of surgical staff behavior on air quality in a conventionally ventilated operating theatre during a simulated arthroplasty: a case study at the University Hospital of Parma. Int J Environ Res Public Health. 2020;17(2):452. https://doi.org/10.3390/ijerph17020452
Raghav N, Mamta, Shrivastava JN, Satsangi GP, Kumar R. Enumeration and characterization of airborne microbial communities in an outdoor environment of the city of Taj, India. Urban Clim. 2020;32:100596. https://doi.org/10.1016/j.uclim.2020.100596
Suehara MB, Silva MCP. Prevalência de fungos anemófilos no Brasil e a correlação com doenças respiratórias e infecções fúngicas. Ciênc Saúde Coletiva. 2023;28(11):3289-300. https://doi.org/10.1590/1413-812320232811.08302022
Houbraken J, Samson RA. Phylogeny of Penicillium and the segregation of Trichocomaceae into three families. Stud Mycol. 2011;70(1):1-51. https://doi.org/10.3114/sim.2011.70.01
Sobral LV. Fungos anemófilos de ambiente hospitalar: perfil de suscetibilidade antifúngica e alergenicidade [tese]. Recife: Universidade Federal de Pernambuco, Programa de Pós-Graduação em Biologia de Fungos; 2023.
Chang CC, Ananda-Rajah M, Belcastro A, McMullan B, Reid A, Dempsey K, et al. Consensus guidelines for implementation of quality processes to prevent invasive fungal disease and enhanced surveillance measures during hospital building works, 2014. Intern Med J. 2014;44(12b):1389-97. https://doi.org/10.1111/imj.12601
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2025 Amanda Corrêa Rocha Bortoli, Cibele Cristina Tramontini, Karine Silva de Oliveira, Amanda Denobi Galassi , Eliana Carolina Vespero, Pedro Olimpio Siqueira Castilho
This work is licensed under a Creative Commons Attribution 4.0 International License.
By publishing in Revista SOBECC, authors retain the copyright of their article and agree to license their work using a Creative Commons Attribution (CC BY 4.0) International Public License, thus accepting the terms of this license. The CC BY 4.0 license allows others to distribute, remix, adapt, and create from the published article, even for commercial purposes, provided they give due credit to the creators of the work (authors of the article).
The authors grant to Revista SOBECC the right of first publication, to identify itself as the original publisher, and grant to the journal a non-exclusive license to use the work in the following ways: (1) to sell and/or distribute the article in hard copies and/or in electronic format; (2) to distribute parts and/or the entire article in order to promote the journal through the internet and other digital and printed media; (3) to record and reproduce the article in any format, including digital media.
With this license, authors can enter into separate contracts for non-exclusive distribution of the article (e.g., publishing in an institutional repository or as a book chapter), with acknowledgement of authorship and initial publication in Revista SOBECC. Authors are encouraged to publish and distribute their work online after publication in the Revista SOBECC, as this can increase the article's visibility and impact.
In line with the journal's policies, each published article will be assigned a CC BY 4.0 license, which will be visible on the abstract page and in the PDF of each article with the respective link to the license terms.
